Semen motility
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Add 100-200 µl of ejaculate to 0.5 ml warmed extender for motility analysis. A water bath is used to keeps samples and extender warm. |
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Make a slide of 10 µl of extended semen under a 18 mm coverslip or 15 µl under a 22 mm coverslip. A slide warmer is used to keep slides, coverslips and pipette tips that come in contact with the semen warm. |
| View the sample to determine total and progressive motility. The sample must be kept warm during viewing as temperature effects motility. | |
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As an alternative to direct viewing on a microscope is to connect it to a video monitor. This is video microscope kit to estimate sperm motility. (Animal Reproduction Systems, 14395 Ramona Avenue, Chino CA 91710) |
