Semen collection and evaluation

University of wisconsin-madison

Semen evaluation

Once the semen is collected, it is time to return to the lab and evaluate the semen before preparation of a sample for artificial insemination. Evaluation will consist of determining the semen:

Volume
Concentration
Motility
Morphology

Step 1

	Remove the gel filter from the collection vial and then pour the gel-free semen into a warmed graduated cyclinder to determine gel-free volume. A typical volume is 45± 30 ml Factors which effect the volume of the ejaculate are: Sexual preparation Time since last ejaculate Season of the year
Step 2
	Remove approximately 1 ml of the semen and place in a small tube to determine sperm concentration and morphology. We recommend that motility only be evaluated on diluted semen. A small sample should therefore be diluted with warm extender to determine motility.
Step 3
	A sample is prepared to determine sperm concentration.
	A sample is preserved to determine the % of sperm with normal morphology

AV
- Colorado
- Missouri
Lab equipment