Place the inner liner in the AV casing so that equal lengths
protrude at each end. The tapered end protrudes from the non-flared
end of the casing.
The liner is straightened so that there are no wrinkles or twists.
The end of the liner is stretched over the flared end of the casing.
Care should be taken when stretching the inner liner since it
may tear if too much force is used.
Disposable, plastic liners may also be used. These liners are
more likely to wrinkle or twist so special care must be taken.
A clamp must be used to secure the liner in place.
The AV is filled with 57°C (135°F) water.
Pull on the tapered end of the liner during filling to assure
that wrinkles and twists do not develop.
After filling, a dial thermometer can be carefully inserted
to monitor the temperature until it stabilized (10-20 sec).
The target temperature is 44 to 50°C but will depend on
the individual stallion and collection conditions.
When the temperature has stabilized, the warmed collection
bottle with a gel filter is clamped on the tapered end of the
liner.
The warmed protector jacket is placed over the collection bottle
and tightened onto the lower end of the casing.