University of wisconsin-madison

Post-thaw Evaluation of Frozen Semen

  1. Evaluate 1 straw for each freezing run per stallion
  2. Evaluate motility when incubated at 37-39°C for 10 and 30 minutes after thawing
  3. If there is some concern about semen quality, thaw another straw.
  4. Discard any ejaculate with poor motility post-thaw
    ( should be >30% at 10 minutes after thawing)
  5. If purchasing frozen semen, some expectation of post-thaw motility should be provided by seller.

next back