University of wisconsin-madison

Artificial Insemination of the Mare

Insemination Procedure

  1. Put lubricant on the knuckles of gloved hand.With the labia lips held apart by an assistant, insert the gloved hand into the vagina at a 35-45° angle.
    Note: Protecting the pipette into the vagina in the palm and then in constant contact with the index finger, will prevent accidental insertion of the pipette into the urethra or the fossa around the cervical os. Perforation of the reproductive tract can be fatal.
  1. Keeping the catheter protected in the palm of your hand, find the cervix. Using your index finger, carefully dilate the cervix. Pass the finger through the cervix into the uterus.
  1. Pass the catheter through the cervix by guiding it along your finger. When the tip of the catheter enters the uterus, remove your finger and hold the cervix closed around the catheter.

  2. Deposit the semen slowly by depressing the plunger of the syringe. Gently hold the cervix closed for 1 minute after depositing the semen. Do not deposit air in the uterus.
  1. Remove your hand and catheter from the vagina. Exam the pipette to make sure semen was deposited. Ultrasound in 12-14 days for pregnancy.

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Artificial Insemination of the Mare