Sperm Preparation

1. Swimup
2. Percoll

Percoll Separation Procedure

Place the Percoll column with the semen layerd on top in the centrifuge. You need to centrifuge at 700 g for 15 minutes. Use the following formula to determine the rpm needed to run the centrifuge.  [Relative Centrifuge Force (G) = (1.119 X 10^-5) X rpm² X radius(cm)]. You must come up with the correct speed before progressing furthe!