Fresh Semen Preparation for Insemination

Initial Steps:

• Remove 1 ml raw semen
• Evaluate semen for concentration, motility and record volume in ml
• Dilute remaining semen 1:1 with warm extender, place on counter.
• Calculate motile sperm /ml in the extended semen (MS/ml)
• MS/ml = (concentration/2)* % motility

Insemination Volume (Inseminate 500 X 10^6 motile sperm in a volume of at least 10 ml):

• Insemination Volume = 500 X 10^6 / (MS/ml)
• Units will be in ml
• If <10 ml then added sufficient extender to insemination dose to bring to 10 ml
• If >10 ml just inseminate volume determined.

Assumptions:

• Hold extended semen at around 21 – 22°C
• place in thermos to ensure semen is not cold shocked
• Insemination to occur within 1 – 2 hours
• If semen will be held 2 – 4 hours, dilute semen at least 1:2 or 1:3
• For longer holding times cool semen following appropriate procedures