| Component | M.W. | mM | amount/100 ml |
| 1. CaCl2.2H2O | 147.02 | 2.0 | 29.4 mg |
| 2. KCl | 74.56 | 3.2 | 23.9 mg |
| 3. MgCl2.6H2O | 203.3 | 0.5 | 10.1 mg |
| 4. NaH2PO4.H2O | 137.99 | 0.4 | 5.5 mg |
| 5. Lactic Acid (Sodium salt; 60% w/w syrup) | 112.1 | 11.0 | 186 µl |
| 6. NaCl | 58.44 | 114.0 | 666 mg |
| 7. NaHCO3 | 84.01 | 25.0 | 210 mg |
| 8. Na Pyruvate (from stock) | 110.0 | 0.2 | 1.0 ml |
| 9. Penicillamine | 20.0 | PHE Stock (see below) | |
| 10. Hypotaurine | 10.0 | PHE Stock (see below) | |
| 11. Epinephrine | 1.0 | PHE Stock (see below) | |
| 12. BSA (Fraction V) | ------ | 6 mg/ml | 600 mg |
| 13. Gentamycin (from stock) | ------ | 50 µg/ml | 100 µl |
| Add approximately 80 ml double distilled water to a beaker. With stiring add components 1 - 7 in order. Add water to bring to 100 ml. This can be stored up to two weeks as a stock at 5 C if filtered and kept sterile. On day of use, add components 8, 12, 13. Note that these can be for any volume desired for use that day and need not be 100 ml. Generally we make 2 - 5 ml on a day. Filter to sterilize with 0.2 µm filters. To fertilization drops (50 µl) add 2 µl of PHE Stock. See Stock solutions for components 8, 9-11, 11 and 13. |