Semen Extenders

• Egg Yolk Citrate

  	Fraction A (Non-glycerol Extender)	Fraction B (Glycerol Extender)
  Ingredient	Amount	Amount
  NaCitrate.2H2O	1.856 gm	1.856 gm
  Glucose	1.0 gm	1.0 gm
  Water	Fill to 80 ml	Fill to 66 ml
  Glycerol	--------------	14 ml
  Egg Yolk	20 ml	20 ml
  pH	to 7.0 with HCl	to 7.0 with HCl
  Gentamycin Sulfate (optional)	100 mg	100 mg

  
  
• Egg Yolk Tris
  

  	Fraction A (Non-glycerol Extender)	Fraction B (Glycerol Extender)
  Ingredient	Amount	Amount
  Tris	2.422 gm	2.422 gm
  Citric Acid Monohydrate	1.360 gm	1.360 gm
  Glucose	1.0 gm	1.0 gm
  Water	Fill to 80 ml	Fill to 66 ml
  Glycerol	--------------	14 ml
  Egg Yolk	20 ml	20 ml
  pH if needed	to 7.0 with HCl	to 7.0 with HCl
  Gentamycin Sulfate (optional)	100 mg	100 mg

  
  
  
• Skim-Milk Glucose (Equine formulation)
  
  
  

  Ingedient	Amount
  Sanalac (instant nonfat dry milk)	2.4 gm
  Glucose	4.9 gm
  Sodium Bicarbonate (stock sol. = 7.5 gm/100 ml)	2 ml
  Gentamycin Sulfate (optional; stock sol. = 50 mg/ml)	2 ml
  Distilled Water	92 ml
  Adjust pH if needed to 7.0

  
  
  

  Place Sanalac and glucose in a beaker. Add the water and mix until milk and glucose are dissolved. Add the sodium bicarbonate and gentamycin sulfate. Measure the pH and adjust if needed.

  
  
  
• Minnesota Avian Extender (MNA; Tajima et al., 1989; J. Reprod. Fert. 85:1-5)

Ingedient	Amount/liter	Amount/100 ml
Tes	4.0 gm	400 mg
Hepes	4.0 gm	400 mg
Bes	3.0 gm	300 mg
Potassium Hydroxide	1.0 gm	100 mg
Glucose	10.0 gm	1000mg
Sorbitol	0.7 gm	70 mg
Potassium Phosphate Dibasic	7.0 gm	700 mg
Potassium Phosphate Monobasic	1.6 gm	160 mg
Sodium Phosphate Dibasic	0.8 gm	80 mg
Potassium Citrate	0.5 gm	50 mg
Magnesium Sulfate	0.35 gm	35 mg
L-glutamic acid (monopotassium salt)	2.1 gm	210 mg
L-glutamic acid (monosodium salt)	6.0 gm	600 mg
Sodium Acetate	2.5 gm	250 mg
Potassium Acetate	2.1 gm	210 mg
Distilled Water	fill to 1000 ml	fill to 100 ml
Adjust pH if needed to 7.1
Osmotic Pressure = 370 mosmol/kg
MNA Modifications (mMNA) at UW-Madison by J.J. Parrish
Ingredient	Amount	Amount
Replace Bes with Mops	2.95 gm	295 mg

 

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