The University of Wisconsin-Madison Skip navigationUW HomeMy UWUW Search
Animal Sciences > Faculty Index > Crenshaw  
Dept. of Animal Sciences logo

Amin Fadl, Assistant Professor                  


Department of Animal Science;
Animal Science Bldg.
1675 Observatory Drive
Madison, WI 53706
Telephone: (608) 890-2398
Fax: 608.262.5157
email: fadl@wisc.edu
CV

PubMed citations

Amin Fadl

Education Research Committees Teaching Publications Useful Links

Education:

B V.Sc,Veterinary Medicine, University ofKhartoum, Khartoum, Sudan.
MS, Microbiology, University of Conneticut, Storrs, CT.

Ph.D., Microbiology, University of Conneticut, Storrs, CT.
Postdoctoral Fellow, Microbiology, University of Texas Medical Branch at Galveston.

Research Interests:

Prevalence of Salmonella in retail poultry, beef and dairy products remains a significant public health concern. Salmonella is the most reported food-borne pathogen causing an estimated 1.4 million cases of salmonellosis with annual economic loss to the U. S. food industry and medical expenses exceeding $1 billion. Upon ingestion of contaminated food, Salmonella rapidly penetrate intestinal mucosa by transcytosis through M cells and enterocytes and enter mononuclear and dendritic cells in Peyer’s patches or taken up by macrophages and carried to the mesenteric lymph nodes reaching internal organs such as liver and spleen. Our research interests focus on the molecular pathogenesis of Salmonella and host-pathogen interaction using cell culture system and animal model of infection. Our efforts are aimed at the pre-harvest aspect of food-safety specifically prevention of Salmonella initial phase of infection such as entry and invasion of host intestinal mucosa. Braun (murein) lipoprotein (Lpp) is a major component of the outer membrane of gram-negative enteriobacteriaceae and functions as a potent stimulator of inflammatory and immune responses resulting in septic shock. Our hypothesis is that Lpp significantly contributes to the virulence of Salmonella and in modulating signal transduction pathway(s) in the host during disease process. Lpp deletion mutant of Salmonella Typhimurium (lppAB) was avirulent in mice and mice immunized with the mutant were completely protected from lethal challenge of the wild-type. On the other hand, Salmonella Enteritidis (SE) infection in humans is caused by contaminated chicken products (meat & eggs). SE often does not cause symptoms in infected chickens; instead it infects the intestinal track and silently spreads through fecal shedding. SE has a specific set of genetic factors responsible for infection and colonization of the intestinal tract of hosts. Identification and characterization of these factors involved in initiation of infection in chickens is one of our research goals. Such bacterial factors and mechanisms of infection could be used as a control and therapeutic targets to reduce the spread of Salmonella in food animals and human population. Further, previous studies have indicated potential involvement of several genes as global regulator of Salmonella virulence. As a result, we developed several Salmonella mutant strains to study the role of these genes during Salmonella infection. Also, we are interested in host cell signaling triggered by bacterial component such as endotoxins and enterotoxins. The long-term goal of our research includes development of attenuated strains of Salmonella that could be used as live attenuated vaccine to protect animals and ultimately humans against salmonellosis.

Courses Taught:

Current:

      Previous:

Awards:

Professional Societies:

Other Responsibilities /Committees

University

Publications:

Useful Links:

1. USDA National Animal Disease Center: http://ars.usda.gov/main/site_main.htm?modecode=36253000
2. National Institute of Allergy & Infectious Diseases/NIH:
http://www3.niaid.nih.gov/
3. Salmonella information and resources for researchers
http://www.salmonella.org/
4. U.S. Food & Drug Administration:
http://www.fda.gov/
5. Center for Disease Control & Prevention:
http://www.cdc.gov/

 



Animal Sciences Animal Sciences | UW Home