Amin Fadl, Assistant Professor
Department of Animal Science;
B V.Sc,Veterinary Medicine, University ofKhartoum, Khartoum, Sudan.
MS, Microbiology, University of Conneticut, Storrs, CT.
Ph.D., Microbiology, University of Conneticut, Storrs, CT.
Postdoctoral Fellow, Microbiology, University of Texas Medical Branch at Galveston.
Prevalence of Salmonella in retail poultry, beef and dairy products remains a significant public health concern. Salmonella is the most reported food-borne pathogen causing an estimated 1.4 million cases of salmonellosis with annual economic loss to the U. S. food industry and medical expenses exceeding $1 billion. Upon ingestion of contaminated food, Salmonella rapidly penetrate intestinal mucosa by transcytosis through M cells and enterocytes and enter mononuclear and dendritic cells in Peyer’s patches or taken up by macrophages and carried to the mesenteric lymph nodes reaching internal organs such as liver and spleen. Our research interests focus on the molecular pathogenesis of Salmonella and host-pathogen interaction using cell culture system and animal model of infection. Our efforts are aimed at the pre-harvest aspect of food-safety specifically prevention of Salmonella initial phase of infection such as entry and invasion of host intestinal mucosa. Braun (murein) lipoprotein (Lpp) is a major component of the outer membrane of gram-negative enteriobacteriaceae and functions as a potent stimulator of inflammatory and immune responses resulting in septic shock. Our hypothesis is that Lpp significantly contributes to the virulence of Salmonella and in modulating signal transduction pathway(s) in the host during disease process. Lpp deletion mutant of Salmonella Typhimurium (lppAB) was avirulent in mice and mice immunized with the mutant were completely protected from lethal challenge of the wild-type. On the other hand, Salmonella Enteritidis (SE) infection in humans is caused by contaminated chicken products (meat & eggs). SE often does not cause symptoms in infected chickens; instead it infects the intestinal track and silently spreads through fecal shedding. SE has a specific set of genetic factors responsible for infection and colonization of the intestinal tract of hosts. Identification and characterization of these factors involved in initiation of infection in chickens is one of our research goals. Such bacterial factors and mechanisms of infection could be used as a control and therapeutic targets to reduce the spread of Salmonella in food animals and human population. Further, previous studies have indicated potential involvement of several genes as global regulator of Salmonella virulence. As a result, we developed several Salmonella mutant strains to study the role of these genes during Salmonella infection. Also, we are interested in host cell signaling triggered by bacterial component such as endotoxins and enterotoxins. The long-term goal of our research includes development of attenuated strains of Salmonella that could be used as live attenuated vaccine to protect animals and ultimately humans against salmonellosis.
- AS/DS 375 Animal Health & Disease Management (Spring semester)
- Medical Microbiology MBIO 0411 (Bacteriology): Lectures and laboratory course for professional Veterinary Medical and graduate students, Tuskegee University College of Veterinary Medicine (2006-2008).
- Immunology MBIO 0413: Lecture and laboratory course for professional Veterinary Medical and graduate students, Tuskegee University College of Veterinary Medicine.
- Clinical microbiology SMED 0695 (Course Coordinator): for Veterinary Medical Students, Tuskegee University, Alabama (2006-2008).
- Molecular Biology MBIO 0311: Signal Transduction in Health and Disease. Course for professional veterinary Medical students, Tuskegee University College of Veterinary Medicine (2006-2008).
- Medical Microbiology (laboratory course for medical students), University of Texas Medical Branch, Galveston (Spring 2005).
- Principles of Pathobiology PATH 297: Department of Pathobiology, University of Connecticut (spring 1997-1999).
- Histologic Structure and Function (PATH 296): Department of Pathobiology, University of Connecticut (Fall 1997-1999).
- Physiology and Anatomy of Domestic Animals PATH 200: Department of Pathobiology, University of Connecticut (Fall 1997-1999).
- Health & Disease management of Animals PATH 202: Department of Pathobiology, University of Connecticut (spring 1997-1999)
- J. McClaughlin postdoctoral fellowship award in Infection and Immunity, 2004-2006.
- Summer fellowship, University of Connecticut, 1999.
- University of Connecticut pre-doctoral fellowship, 98/1999.
- Summer fellowship, University of Connecticut, 1998.
- University of Connecticut pre-doctoral fellowship, 97/1998.
- University of Connecticut pre-doctoral fellowship, 96/1997.
- Ph. D. Dissertation fellowship, University of Connecticut, summer 1997.
- American Society for Microbiology (ASM)
- American Association for the Advancement of Science (AAAS
- International Association for Food Protection (IAFP))
Other Responsibilities /Committees
- Faculty Senator
- Science & Medicine Graduate Research Scholars (SciMed GRS)
- Sha J, S. L. Agar, W. B. Baze, J. P. Olano, A. A. Fadl, T. E. Erova, S. Wang, S. M. Foltz, G. Suarez, V. L. Motin, S. Chauhan, G. R. Klimpel, J.W. Peterson, A. K. Chopra. 2008. Braun Lipoprotein (Lpp) Contributes to the Virulence of Yersiniae: Potential Role of Lpp in Inducing Bubonic and Pneumonic Plague. Infect. Immun. 76: 1390-1409.
- Erova, T. E., V. G. Kosykh, A. A. Fadl, J. Sha, A. J. Horneman, A. K. Chopra. 2008. Cold Shock Exoribonuclease R (VacB) Is Involved in Aeromonas hydrophila Pathogenesis. J. Bacteriol. 190: 3467-74.
- Salih S.M., S. A. Salama, M. Jamaluddin, A. A Fadl, L. J. Blok, C. W. Burger, M. Nagamani, A. Al-Hendy. 2008. Progesterone-Mediated Regulation of Catechol-O-Methyl Transferase Expression in Endometrial Cancer Cells. Reprod. Sci. 15: 210-220.
- Suarez, G., J.C. Sierra, J. Sha, S. Wang, T.E. Erova, A.A. Fadl, S. M. Foltz, A. J. Horneman, A. K. Chopra. 2008. Molecular characterization of a functional type VI secretion system from a clinical isolate of Aeromonas hydrophila. Microb Pathog, 44: 344-361.
- Erova, T. E., J. Sha, A. J. Horneman, M. A. Borchardt, B. K. Khajanchi, A. A. Fadl and A. K. Chopra. 2007. Identification of a new hemolysin from diarrheal isolate SSU of Aeromonas hydrophila. Gene. (In press).
- Silver, A.C., Y. Kikuchi, A. A. Fadl, J. Sha, A. K. Chopra, and J. Graf. 2007. Interaction between innate immune cells and a bacterial type III secretion system in mutualistic and pathogenic associations. Proc. Nat. Acad. Sci. USA. 104: 9481-9486.
- Salih S. M., S. A. Salama, A. A. Fadl, M. Nagamani, and A. Al-Hendy. 2007. Expression and cyclic variations of catechol-O-methyl transferase in human endometrial stroma. Fertil Steril. (In press).
- Salih, S. M., M. Jamaluddin, S. A. Salama, A. A. Fadl, M. Nagamani, and A. Al-Hendy. 2007. Regulation of catechol-O-methyl transferase (COMT) expression in granulosa cells: a potential role for follicular arrest in polycystic ovary syndrome. J. Clinical Endocrinology & Metabolism. (In Press).
- Sha, J., S.F. Wang, G. Suarez, J. C. Sierra, A. A. Fadl, T. E. Erova, S. M. Foltz, B. K. Khajanchi, A. C. Silver, J. Graf, C. H. Schein, and A. K. Chopra. 2007. Further characterization of a type III secretion system (T3SS) of a new effector protein from a clinical isolate of Aeromonas hydrophila. Microbial Pathogenesis. 43: 127-146.
- Fadl, A. A., C. L. Galindo, J. Sha, H. R. Garner, H. Q. Wang, and A. K. Chopra. 2007. Global Transcriptional Responses of Wild-type Aeromonas hydrophila and its Virulence-deficient Mutant in a Murine Model of Infection. Microbial Pathogenesis, 42: 193-203.
- Ramana, K.V., A. A. Fadl, A. B. Reddy, R. Tammali, A. K. Chopra, and S. K Srivastava. 2006. Aldose reductase mediates the lipopolysaccharide–induced release of inflammatory mediators in RAW264.7 murine macrophages. J. Biological Chemistry 281: 33019-29.
- Erova, T., A. A. Fadl, J. Sha, B. K. Khajanchi, L. Pillai, and A. K. Chopra. 2006. Mutations within the catalytic motif of DNA adenine methyltransferase (Dam) of Aeromonas hydrophila reverts the virulence of Dam-overproducing strain to that of the wild-type phenotype. Infection and Immunity, 74: 5763-72.
- Pillai, L., J. Sha, T. Erova, A. A. Fadl, B. Khajanchi, and A. K. Chopra. 2006. Molecular and functional characterization of a ToxR-regulated lipoprotein from a clinical isolate of Aeromonas hydrophila. Infection and Immunity. 74: 3742-3755.
- Fadl, A. A., C. L. Galindo, J. Sha, T. E. Erova, J. P. Olano, and A. K. Chopra. 2006. Deletion of the genes encoding the type III secretion system and cytotoxic enterotoxin alters host responses to Aeromonas hydrophila infection. Microbial Pathogenesis, 40: 198-210.
- Fadl, A. A., C.L. Galindo, J. Sha, G. R. Klimpel, and A. K. Chopra. 2006. Global gene expression and protein profiling of a murein (Braun) lipoprotein mutant of Salmonella enterica serovar Typhimurium. Gene, 374(7): 121-127.
- Erova, T. E., L. Pillai, A. A. Fadl, J. Sha, S. Wang, C. L. Galindo, and A. K. Chopra. 2006. DNA Adenine methyltransferase influences virulence of Aeromonas hydrophila. Infection and Immunity, 74: 410-424
- Galindo, C. L., J. Sha, A. A. Fadl, L. Pillai, and Ashok. K. Chopra. 2006. Host Immune Responses to Aeromonas Virulence Factors. Current Immunology Review. 3: 13-26.
- Fadl, A. A., J. Sha, G. R. Klimpel, J. P. Olano, C. Galindo, and A. K. Chopra. Attenuation of Salmonella enterica serovar Typhimurium by altering biological functions of murein lipoprotein and lipopolysaccharide. Infection and Immunity, 73: 8433-84436.
- Chopra, V., A. Fadl, J. Sha, C. L. Galindo, V. L. Popov and A. K Chopra. 2005. Alterations in the virulence potential of enteric pathogens and bacterial-host cell interactions under simulated microgravity conditions. J. Tox. Environ. Health. 69: 1345-1370.
- Galindo, C. L., A. A, Fadl, J. Sha, L. Pillai, C. Gutierrez, and A. K. Chopra. Microarray and proteomics analyses of human intestinal epithelial cells treated with Aeromonas hydrophila cytotoxic enterotoxin. Infect. Immun. 73: 2628-43
- Sha, J., L. Pillai, A. A. Fadl, C. L. Galindo, T. E. Erova, V. L. Popov, and A. K. Chopra. 2005. Type III secretion system and cytotoxic enterotoxin alter the virulence of Aeromonas hydrophila. Infect. Immun. 73: 6446-6457.
- Galindo, C. L., A. A. Fadl, J. Sha, C. Gutierrez V. L. Popov, I. Boldogh, B. B. Agarwal, and A. K. Chopra. 2004. Aeromonas hydrophila cytotoxic enterotoxin of activates mitogen-activated protein kinases and induces apoptosis in murine macrophages and human intestinal epithelial cells. J. Biological Chemistry. 279: 37597-37612.
- Fadl, A. A., J. Sha, G. R. Klimpel, J. P. Olano, D. W. Niesel, and A. K. Chopra. 2005. Murein lipoprotein is a critical outer membrane component involved in Salmonella enterica serovar Typhimurium systemic infection. Infect. & Immun. 73: 1081-1096.
- Galindo, C. L., A. A. Fadl, J. Sha, and A. K. Chopra. 2004. Microarray analysis of Aeromonas hydrophila cytotoxic enterotoxin-treated primary peritoneal macrophages. Infect. Immun. 72: 5439-5445.
- * Sha, J., A. A. Fadl, G. R. Klimpel, D. W. Niesel, V. L. Popov, and A. K. Chopra. 2004. The two lipoproteins of Salmonella enterica serovar Typhimurium contribute to the virulence of the organism. Infect. Immun. 72: 3987-4003. (* Equal contribution)
- Sha, J. E. V. Kozlova, A. A. Fadl, J. P. Olano, C. W. Houston, J. W. Peterson, and A. K. Chopra. 2004. Molecular characterization of glucose-inhibited division gene, gidA, that regulates cytotoxic enterotoxin of Aeromonas hydrophila. Infect. Immun. 72: 1084-1095.
- Galindo, C. L., J. Sha, D. A. Ribardo, A. A. Fadl, L. Pillai, and A. K. Chopra. 2003. Identification of Aeromonas hydrophila cytotoxic enterotoxin-induced genes in macrophages using microarrays. J. Biol. Chem. 278: 40198-40212
1. USDA National Animal Disease Center: http://ars.usda.gov/main/site_main.htm?modecode=36253000
2. National Institute of Allergy & Infectious Diseases/NIH:
3. Salmonella information and resources for researchers
4. U.S. Food & Drug Administration:
5. Center for Disease Control & Prevention:
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File last updated: November 11, 2008