Introduction to Practical Microscopy
Animal Sciences 660 / AHABS 660 / Anatomy 660
Room 236 Animal Sciences, 1:00 to 5:00PM, MTWRF
Primary Instructor
Ralph Albrecht Professor, Animal Sciences, 263-3952
WEEK 1, 6/17 through 6/21
Introduction to Scanning Electron Microscopy
WEEK 2, 6/24 through 6/28
Introduction to Transmission Electron Microscopy
WEEK 3, 7/1 through 7/5
Specialized and Ancillary Techniques for EM and Micro CAT Imaging
WEEK 4, 7/8 through 7/12
Introduction to Correlative and Photon-Based Microscopy
WEEK 5, 7/15 through 7/19
Additional Specialized Topics, Final Exam, Begin Optional Projects
WEEKS 6 through 8
Optional Projects / Specialized Training
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Monday
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Tuesday
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Wednesday
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Thursday
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Friday
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June 17
Albrecht/Oshel
Course Introduction; SEM: Theory and practice, modes of operation, types of information obtainable
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June 18
Albrecht
SEM: Specimen Preparation 1
Specimen choice, limitations, fixation, stabilization, dehydration, freezing & freeze-drying, critical point procedure
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June 19
Albrecht
SEM: Specimen Preparation 2
Alternative preparation procedures, mounting, sputter coating, evaporative coating, storage
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June 20
Albrecht/Oshel
Introduction to labeling for SEM; Demo/discussion
Operation of the SEM in SE, BSE, AEI, & EDX modes
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June 21
Albrecht/Oshel
Demo/discussion
Operation of ancillary instrumentation for preparation;
Demo/discussion Operation of high-resolution FESEM
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June 24
Albrecht/Malecki
TEM: Theory and practice, modes of operation, types of information obtainable
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June 25
Oshel
TEM: Specimen Preparation 1
Fixation, dehydration, and staining
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June 26
Oshel
TEM: Specimen Preparation 2
Embedding, embedding agents, sectioning, and post-embedding staining
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June 27
Oshel/Albrecht/Malecki
Demo/discussion
Embedding, sectioning, and staining
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June 28
Oshel/Meyer/Malecki
Demo/discussion
Operation of the TEM
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July 1
TBA
Meyer/Malecki
Energy Filtering TEM - theory and practice, advantages, specialized use in biology, Demo of EFTEM;
Demo of Ion Beam Sputter Coating
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July 2
Oshel/Malecki/Albrecht
Cryomicroscopy, freezing, high pressure freezing, freeze fracture, cryo-imaging.
Demo/Disc HP freezing, cryo-imaging
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July 3
Weichert
(meet at CSC, Room TBA)
micro CAT, micro PET, micro MRI
Demo of Micro CAT
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July 4
Independence Day
Vacation
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July 5
Open
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July 8
Albrecht
Intro to Correlative Microscopy
LM incl phase, interference, video, deconvolution. TEM incl. ivem, hvem, eftem. SEM incl. fesem, lvsem, edx & AFM
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July 9
Albrecht
High resolution immuno-labeling and ligand-labeling for EM and Correlative microscopy (photon, electron, and force-based imaging)
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July 10
Squirrell
Intro to Confocal and Multiphoton Microscopy
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July 11
Eliceiri
Digital imaging, image storage and transmission, remote microscopy
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July 12
Wilsman
Morphometry & stereology
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July 15
Carpick
Building & Room TBA
Scanning Force Microscopy, including STM and AFM, biological applications
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July 16
Fournelle/Noll/Oshel/ Malecki/Albrecht
(Building and Room TBA)
Analytical EM, X-ray microanalysis
Demo of EDX
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July 17
Albrecht/Oshel
Autoradiography
Review, questions, maybe answers, and repeat demos
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July 18
Albrecht/Oshel
EXAM
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July 19
Albrecht/Oshel
Discuss optional projects
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NOTE:
We have attempted to organize the course in week long areas of topical emphasis as much as the schedules of participating faculty have permitted. Each area consists of roughly 20 hours of lecture and laboratory demonstration covering the fundamentals of the particular area. Both theoretical and practical aspects are addressed.
Students, staff, and faculty in the Center for Developmental and Molecular Toxicology are invited to sit in on specific topics which may be of interest to them.
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