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Instrument and Service Fees for UW Madison Researchers*

Personnel Time $44 per hour
Training Personnel time plus instrument use if applicable
Hitachi S-3200 SEM $45 per hour
Hitachi S-900 FE-SEM $45 per hour
Zeiss Confocal Microscope $32 per hour
Sputter Coater $25 per run
Critical Point Dryer $25 per run
Cryocoater $50 per run
Ion-Beam Coater $50 per run
RMC Ultramicrotome $25 per run
Chemical Cost $40 per run
Chemical Cost (if not imaged) $50 per use
Conjugating Antibodies/Ligands UW-Madison campus users: personnel time
other non-profit and educational users: personnel time + cost of the conjugate(s)
*Non-UW-Madison users contact Fikrullah Kisa for costs



Training is provided for all instruments and procedures except for the high-resolution Hitachi S-900 field emission SEM. All training is done as a one-on-one tutorial, although small groups from a single lab can also be accommodated. The details and time necessary vary from instrument to instrument and the experience of the people being trained, but generally the preparatory equipment takes one to two sessions, the Zeiss light microscope one session of about 3 hours, and the Hitachi S-570 SEM 2 or 3 sessions. Making colloidal metals and conjugating them to proteins or other ligands generally involves a half-day, but difficult conjugates and metals other than gold can take longer, maybe a couple of days.

It is preferable to do the training using the investigator's samples, but it is also a good idea to start with samples that are not particularly important. The first try either works perfectly or fails horribly.